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Pioneering Technology

Accuracy and quantification by design

immunoSEQ Assays utilize a multiplex PCR-based assay enabling us to sequence directly from genomic DNA. We provide precise, quantitative abundance data on your cell populations, unlike the relative abundance or variable expression data of RNA-based assays. Each immunoSEQ Assay contains rigorously designed synthetic immune templates as in-line controls plus optimized primers that ensure accurate, quantitative, and unbiased results with batch-to-batch consistency.

1
First, a synthetic immune repertoire was designed to represent all VJ-gene combinations.

2
Then, measuring against our synthetic repertoire, we were able to adjust our primer concentrations to significantly reduce amplification bias.

3
In-line controls for every sample enable us to measure amplification, correct residual bias, and provide absolute counts of the immune cells in your sample.

4
Our sophisticated bioinformatics platform corrects errors and collapses sequences to deliver highly accurate, high volume, standardized data sets.

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